Browsing by Author "Amarawansha, T.W.G.J.C ."

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    Development of micropropagation protocols for two aponogeton species of Sri Lanka (Aponogeton crispus and Aponogeton natans)
    (Uva Wellassa University of Sri Lanka, 2015) Amarawansha, T.W.G.J.C .; Bambaranda, B.V.A.S. M.; Jayamanne, S.C.; Herath, H.M.I
    Sri Lanka being a tropical country contributes for a highly diversified flora and fauna. These climatic conditions have become a critical factor for the occurrence of highly diversified aquatic flora as well. Aponogeton is genus of aquatic plants which belongs to the family Aponogetonaceae. Methodology The study was carried out at tissue culture laboratory of Royal Botanic Gardens, Peradeniya. Mother plants were collected from different areas of Sri Lanka. Experiments were done to determine hormonal effect on shoot initiation and multiplication using rhizomes of Aponogeton crispus and A. jacobsenii; leaf and leaf stalks of A. natans and A. jacobsenii and seeds of A. crispus and A. natans. For the Sterilization of A. crispus and A. jacobsenii rhizomes were kept under running tap water for 3 hours and dipped in a fungicide for 30 minutes. Then disinfected using 20 % NaOCl (Clorox) with 2 drops of Tween twenty for 10 minutes and washed with 0.1 % HgCl2 for 7 minutes. Finally rhizomes were washed 5 times thoroughly with distilled water per 5 minutes. For seeds sterilization seeds were kept under running tap water for 10 minutes, disinfected using 5 % Clorox with 2 drops of Tween twenty for 15 minutes and washed 3 times with distilled water. Three sterilization procedures were carried out for leaves and leaf stalks. Firstly leaves kept under running tap water for 1 ½ hours and dipped in fungicide (topsin) for half an hour.